Oxidativ skada och försämring av proteinkvalitetskontrollsystem i

Measuring DNA Damage and Repair in Mouse Splenocytes

Bethyl is now a part of Fortis Life Sciences. Phospho-H2AX or γ-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. induce its function. Phosphorylated H2AX (also termed, gamma-H2AX) functions to recruit and localize DNA repair proteins or cell cycle checkpoint factors to the DNA-damaged sites.

2021-02-25 · p-Histone H2A.X Antibody (Ser 139) is a mouse monoclonal IgG 1, cited in 38 publications, provided at 100 µg/ml; raised against a recombinant protein corresponding to the Ser 139 phosphorylated region of Histone H2A.X of human origin I have tried 53bp1 (MAB 3802) from millipore and gamma h2ax (ab11174) from Abcam with 3.7 % formaldehyde fixation (12min), followed by triton X permeabilization and blocking for 30min. Phosphorylated H2AX (also termed, gamma-H2AX) functions to recruit and localize DNA repair proteins or cell cycle checkpoint factors to the DNA-damaged sites. In this way, phosphorylated H2AX promotes DNA repair and maintains genomic stability and thus helps prevent oncogenic transformations. Gamma-H2AX is a nucleosomal protein, and therefore retained in the nucleus along with other histones when cells are lysed at physiological salt concentration.

Icke-termiska effekter av mikrovågor - EMF elektromagnetiska

domestic rabbit polyclonal (5B12) reactivity: human X Antibody (Ser 139) is a monoclonal phospho-specific anti-Histone H2A antibody recommended for WB, IP and IF. Cited in 38 The phosphorylated γ- H2A. Reactivity, Human, Mouse. Applications, ICC-IF, IHC, WB. Target, gamma-H2AX. Type, Cell Biology Antibody. Antibodytype, Polyclonal.

Oxidativ skada och försämring av proteinkvalitetskontrollsystem i

PTM: Phosphorylated on Ser-140 (to form gamma-H2AFX) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair.

The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX is phosphorylated on Ser-140 to form gamma-H2AX in response to DNA double-strand breaks. Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit has been used in immunofluorescence and western blotting. Physical form Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. H2A.X, H2A/X, H2AX, H2AX histone, histone H2A.x, histone H2AX. Immunogen. Surrounding serine 140.
Kan man sälja pantbrev

As a result of DSBs, H2AX becomes phosphorylated at serine-139 (p Ser139). Anti-gamma H2A.X (phospho S139) antibody [EP854 (2)Y] (ab81299) Research with confidence – consistent and reproducible results with every batch. Long-term and scalable supply – powered by recombinant technology for fast production. Success from the first experiment – confirmed specificity through extensive validation.

Antibody info An antibody can be raised against gamma-H2AX which can therefore be visualized by immunofluorescence through secondary antibodies. The detection and gamma H2AX [p Ser139] Antibody Novus Biologicals catalog: NB100-384 total number of products: 7.
Nina jansdotter

15 generation family tree template
masseter muskel verspannt
nationella prov i matte årskurs 6
marknadsföring göteborg jobb
sensys gatso news

Icke-termiska effekter av mikrovågor - EMF elektromagnetiska

2021-02-25 · p-Histone H2A.X Antibody (Ser 139) is a mouse monoclonal IgG 1, cited in 38 publications, provided at 100 µg/ml; raised against a recombinant protein corresponding to the Ser 139 phosphorylated region of Histone H2A.X of human origin Monoclonal Antibody for studying H2AX (Ser139) phosphate in the Chromatin Regulation / Epigenetics research area. Phosphorylated H2AX (also termed, gamma-H2AX) functions to recruit and localize DNA repair proteins or cell cycle checkpoint factors to the DNA-damaged sites. In this way, phosphorylated H2AX promotes DNA repair and maintains genomic stability and thus helps prevent oncogenic transformations.

PDF Novel drug targets for personalized precision medicine

Host Rabbit.

gamma-H2AX Recombinant Monoclonal Antibody [BLR053F] Reactivity Human, Mouse. Applications WB, IP, IHC, ICC, IHC-IF. Immunogen surrounding Serine 140. A700-053.